USP 171 Vitamin B12 Activity Assay

光风霁月

USP 171 Vitamin B12 Activity Assay
全文见附件，疑问如下：
1. 在Assay Preparation中，Autoclave the mixture at 121° for 10 minutes的目的是控制生物负载 还是 其他如促进转化目的？
2. 在Procedure中，Cover the tubes suitably to prevent bacterial contamination, and sterilize the tubes and contents in an autoclave at 121° for 5 minutes, arranging to reach this temperature in not more than 10 minutes by preheating the autoclave, if necessary. Cool as rapidly as practicable to avoid color formation resulting from overheating the medium.此处121℃保持5min，是不是算上升温降温段达到累计F0≥8min还是 只是消毒目的？
有没有了解的，望解答。

光风霁月

查阅了与《USP 171 Vitamin B12 Activity Assay》中测定方法类似的《GB 5413.14-2010 食品安全国家标准婴幼儿食品和乳品中维生素B12的测定》，确认这两个处理过程目的：
1、在Assay Preparation中，Autoclave the mixture at 121° for 10 minutes 目的是水解；
2、在Procedure中，Cover the tubes suitably to prevent bacterial contamination, and sterilize the tubes and contents in an autoclave at 121° for 5 minutes, arranging to reach this temperature in not more than 10 minutes by preheating the autoclave, if necessary. Cool as rapidly as practicable to avoid color formation resulting from overheating the medium. 此处121℃保持5min（商品培养基按标签说明进行灭菌）目的是灭菌（残存概率法）。

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